- Structure-function relationship of proteins
- Enzyme mechanism
- Biological properties of proteins
Glu I is the first enzyme to act in processing oligosaccharides N-linked to proteins. While Glu I essential for normal cell biochemistry and viability in higher organisms, there is solid evidence that inhibitors of this enzyme can be effective therapeutic agents to treat disease states that manifest in abnormal glycosylation or rely on glyco-recognition.
T/PALs are one of the few non-hydrolytic enzymes that have considerable industrial application. The enzyme from different sources show very different specificity for either Phe or Tyr, and this is being investigated to develop enzymes with more useful characteristics.
Tinamous are terrestrial birds endemic to South America with commercial potential in British Columbia. There is very little information available on the protein composition of tinamou eggs. We are investigating their functional and biological properties.
Microfluidic systems miniaturize analyses, which allows results to be rapidly generated on-site. This makes it an ideal approach for many food analyses. We are investigating the use of molecularly imprinted polymers as a solid phase extraction medium to use in a microfluidics system.
Undergraduate: FNH 325/326 – Food Science Laboratory I/II – Integrated laboratory encompassing the processing and analysis of foods.
- FOOD 522 – Advances in Food Chemistry
- FOOD 500/600 – Graduate Seminar
Barker MK, Wilkinson BL, Faridmoayer A , Scaman CH, Fairbanks AJ, Rose DR. 2011. Production and crystallization of processing alpha-glucosidase I: P. pastoris expression and a two step purification toward structual determination. Protein Expression & Purification.
Lam M , Scaman CH, Kermode A, Clemens S. 2008 Retention of phenylalanine ammonia-lyase activity in wheat seedlings during storage and in vitro digestion Journal of Agricultural & Food Chemistry 56, 11407–11412.
Goldson A, Lam M ; Scaman, CH, Clemens S, Kermode A. 2008 Screening of phenylalanine ammonia lyase in plant tissues, and retention of activity during dehydration Journal of the Science of Food & Agriculture, 88, 619-625.
Kozak M, Scaman, CH 2008 Unsupervised Classification Methods in Food Sciences: Discussion and outlooks. Journal of the Science of Food & Agriculture, 88, 1115-1127.
Kim H, Hartnett C, Scaman CH. 2007 Free galactose content in selected fresh fruits and vegetables, and soy beverages. Journal of Agricultural & Food Chemistry 55, 8133-8137.
Faridmoayer A, Scaman CH. 2007 Truncations and functional carboxylic acid residues of yeast processing alpha-glucosidase I. Glycoconjugate Journal 24, 429-437.
Scaman CH, Nakai S, Aminlari M. 2006 Effect of pH, temperature and sodium bisulfite or cysteine on the level of Maillard-based conjugation of lysozyme with dextran, galactomannan and mannan Food Chemistry, 99(2) 368-380.
Faridmoayer A, Scaman, CH. 2005 Binding residues and catalytic domain of Saccharomyces cerevisiae processing alpha-glucosidase I. Glycobiology 15, 1341-1348.
Faridmoayer A, Scaman, CH.. 2004 An improved purification procedure for soluble processing alpha-glucosidase I from Saccharomyces cerevisiae overexpressing CWH41. Protein Expression & Purification. 33:11-18
Scaman CH, Jim VJ, Hartnett C. 2004 Free galactose concentrations in fresh and stored apples (Malus domestica) and processed apple products. Journal of Agricultural & Food Chemistry. 52:511-7.
Dhanawansa R, Faridmoayer A, van der Merwe G, Li YX, and Scaman CH. 2002 Overexpression, purification, and partial characterization of Saccharomyces cerevisiae processing alpha glucosidase I. Glycobiology, 12, 229-234.
- Venkatrao Konasani (PhD student) – Structure and mechanism of alpha processing glucosidase I
- Alireza Riazi (PhD student) – Microfluidic analysis of vitamins using molecularly imprinted polymers.
- Andrea Goldson (PhD student) – Tyrosine/phenylalanine ammonia lyase cloning and characterization
- Reena Mistry (MSc student) – Optimization of molecularly imprinted polymer formation
- Orly Varon (MSc student) – Timamou egg white protein characterization and antimicrobial properties
- Amandine Arenas (Visiting student Ensbana, France) May – Oct 2012
- Camille Bertolo (Visiting student Ensbana, France) May – Oct 2012